Complement Factor H anticorps (Internal Region)

N° du produit ABIN185437

Cet anticorps Chèvre Polyclonal détecte spécifiquement Complement Factor H dans WB, IHC, ELISA et FACS. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour Complement Factor H anticorps (Internal Region) (ABIN185437)

Antigène: Complement Factor H (CFH)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Complement Factor H est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-Complement Factor H anticorps

Épitope: Internal Region

Fonction: Complement factor H

Séquence: HLVPDRKKDQ YK

Specificité: This antibody is expected to recognize isoform a (NP_000177.2) only.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Western Blot: Approx 150 kDa band observed in Human Kidney lysates and in lysates of cell line K562 (calculated MW of 139 kDa according to NP_000177.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Complement Factor H (ABIN185437)

Fujisawa, Yasumoto, Kato, Sugawara, Yoshida, Yatomi, Nangaku: "The role of anti-complement factor H antibodies in the development of atypical haemolytic uremic syndrome: a possible contribution to abnormality of platelet function." dans: British journal of haematology, Vol. 189, Issue 1, pp. 182-186, (2020) (PubMed).

Détails sur Complement Factor H

Antigène: Complement Factor H (CFH)

Autre désignation: CFH

Sujet: Complement factor H , HGNC:4883, CFH, CFHL3, FHL1, HF, HF1, HF2, HUS, H factor 1 (complement), H factor 2 (complement), H factor-1 (complement), factor H-like 1, AHUS1, ARMD4, ARMS1, FH, age-related maculopathy susceptibility 1, beta-1-H-globulin, beta-1H

ID gène: 3075

NCBI Accession: NP_000177

Pathways: Système du Complément, Cellular Response to Molecule of Bacterial Origin