Clusterin anticorps (C-Term)
Aperçu rapide pour Clusterin anticorps (C-Term) (ABIN185475)
Antigène
Voir toutes Clusterin (CLU) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
-
-
Épitope
- C-Term
-
Fonction
- Clusterin / APOJ
-
Séquence
- EKALQEYRKK HREE
-
Specificité
- This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
-
Réactivité croisée
- Chien, Humain
-
Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
Immunogène
- Peptide with sequence C-EKALQEYRKKHREE, from the C Terminus of the protein sequence according to NP_001822.2, NP_976084.1.
-
Isotype
- IgG
-
-
-
-
Indications d'application
-
Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 35-37 kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8 kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID: 9
Peptide ELISA: antibody detection limit dilution 1:64000.
-
Commentaires
-
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 0.5 mg/mL
-
Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Conseil sur la manipulation
- Minimize freezing and thawing.
-
Stock
- -20 °C
-
Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- Clusterin (CLU)
-
Autre désignation
- CLU
-
Sujet
- CLU, APOJ, clusterin, complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J , HGNC:2095, CLI, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2 , apolipoprotein J, clusterin, complement-associ
-
ID gène
- 1191
-
NCBI Accession
- NP_001822, NP_976084
-
Pathways
- Apoptose, Negative Regulation of intrinsic apoptotic Signaling
Antigène
-