FZD8 anticorps (C-Term)

N° du produit ABIN185522

L’anticorps Chèvre Polyclonal anti-FZD8 a été validé pour WB, ELISA, IHC, IF et IEM. Il convient pour détecter FZD8 dans des échantillons de Humain.

Aperçu rapide pour FZD8 anticorps (C-Term) (ABIN185522)

Antigène: FZD8 (Frizzled Family Receptor 8 (FZD8))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FZD8 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)

Classe de qualité: Verified

Détail du produit anti-FZD8 anticorps

Épitope: C-Term

Fonction: FZD8 / frizzled 8

Séquence: SYPKQMPLSQ V

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 3 μg/mL.

Western Blot: Approx 75 kDa band observed in Human Pancreas lysates (calculated MW of 73.3 kDa according to NP_114072.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse: Zhao B e

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602.

Additional validation: This antibody was successfully used in Electron microscopy (immunogold staining):

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur FZD8

Antigène: FZD8 (Frizzled Family Receptor 8 (FZD8))

Autre désignation: FZD8

Sujet: FZD8, frizzled homolog 8 (Drosophila) , HGNC:4046, FZ-8, hFZ8 , frizzled (Drosophila) homolog 8, frizzled 8

ID gène: 8325, 14370

NCBI Accession: NP_114072

Pathways: Signalisation WNT