14-3-3 sigma/SFN anticorps (Internal Region)

N° du produit ABIN185539

Cet anticorps Chèvre Polyclonal détecte spécifiquement 14-3-3 sigma/SFN dans WB, IHC et ELISA. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour 14-3-3 sigma/SFN anticorps (Internal Region) (ABIN185539)

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp 14-3-3 sigma/SFN est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-14-3-3 sigma/SFN anticorps

Épitope: Internal Region

Fonction: 14-3-3 sigma / Stratifin

Séquence: DLHTLSEDSY KDST

Réactivité croisée: Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 26-28 kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8 kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1). Recommended concentration: 0

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur 14-3-3 sigma/SFN

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Autre désignation: SFN

Sujet: SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS

ID gène: 2810, 55948, 313017

NCBI Accession: NP_006133

Pathways: Signalisation p53, Myometrial Relaxation and Contraction