Melanoma gp100 anticorps (C-Term)

N° du produit ABIN185660

L’anticorps Chèvre Polyclonal anti-Melanoma gp100 a été validé pour WB, IF et ELISA. Il convient pour détecter Melanoma gp100 dans des échantillons de Humain et Souris.

Aperçu rapide pour Melanoma gp100 anticorps (C-Term) (ABIN185660)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Melanoma gp100 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Classe de qualité: Verified

Détail du produit anti-Melanoma gp100 anticorps

Épitope: C-Term

Fonction: Silver homologue / Pmel 17 (C-terminus)

Séquence: CPIGENSPLL SGQQ

Réactivité croisée: Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence CPIGENSPLLSGQQ, from the C Terminus of the protein sequence according to NP_008859.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 70 kDa band observed in Mouse Skin lysates (calculated MW of 66.3 kDa according to Mouse NP_068682.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of A431cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: PMEL

Sujet: Silver homolog (mouse) , PMEL17, SILV , D12S53E, ME20, SI, SIL, gp100 , Melanocyte protein mel 17, Pmel 17, Silver, mouse, homolog of, silver (mouse homolog) like, silver (mouse homolog)-like, silver homolog

ID gène: 6490, 20431, 362818

NCBI Accession: NP_008859