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SMAD2 anticorps (AA 272-467)

Cet anticorps anti-SMAD2 est un anticorps Lapin Polyclonal détectant SMAD2 dans WB, IHC, IP et ICC. Adapté pour Rat.
N° du produit ABIN1860588

Aperçu rapide pour SMAD2 anticorps (AA 272-467) (ABIN1860588)

Antigène

Voir toutes SMAD2 Anticorps
SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivité

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Rat

Hôte

  • 249
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Lapin

Clonalité

  • 222
  • 46
Polyclonal

Conjugué

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Cet anticorp SMAD2 est non-conjugé

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Épitope

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    AA 272-467

    Fonction

    Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)

    Séquence

    MGHHHHHHSG SEF- AFWCSIAYY ELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGC NLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS

    Specificité

    The antibody is a rabbit polyclonal antibody raised against Smad2. It has been selected for its ability to recognize Smad2 in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    Recombinant Smad2 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against Smad2.

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

    Autre désignation

    Smad2

    Sujet

    JV18, JV18-1, MADH2, MADR2, HMAD-2, HSMAD2, Mad-related protein 2

    Pathways

    Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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