FAS anticorps

N° du produit ABIN187302

Cet anticorps anti-FAS est un anticorps Souris Monoclonal détectant FAS dans FACS, IP, IHC (fro) et Func. Adapté pour Humain.

Aperçu rapide pour FAS anticorps (ABIN187302)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 2R2

Détail du produit anti-FAS anticorps

Specificité: This antibody is specific for human Fas. Species Reactivity: Human. Other species not tested.

Immunogène: Mouse L cells stably over-expressing human Fas.

Isotype: IgG3

Information d'application

Indications d'application: Functional activity: Induces apoptosis, no cross-linking required. Can be used in DISC (death-inducing signaling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation. Flow cytometry The optimal dilution for a specific application should be determined by the researcher. Figure 1. Induction of apoptosis in anti-Fas 2R2 stimulated Jurkat T-cells. Method: (A) Dose response: Jurkat cells (1x105) were treated with agonistic anti-Fas 2R2 antibody at the indicated concentrations for 24 h. Induction of apoptosis was assessed by PI staining of hypodiploid nuclei and subsequent flow cytometry. (B) Time course of apoptosis: Jurkat cells (1x105) were treated with 1 µg/mL anti-Fas 2R2. After the indicated time points apoptosis was determined by FACS measurement of hypodiploid DNA. Figure 2. Detection of Fas surface expression by FACS analysis. Method: Normal L cells (open histogram) and cells stably over-expressing human Fas (filled histogram) were incubated with anti-Fas 2R2 and analyzed by indirect immunofluorescence using PE-labeled secondary antibodies. No immunostaining was obtained with an isotype matched IgG control.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 µg monoclonal antibody at 1 mg/mL purified from concentrated hybridoma tissue culture supernatant. Prepared in PBS containing protein stabilizer. Protein A-affinity purified. Purity 95% by SDS-PAGE.

Stock: 4 °C

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: CD95 / FAS

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity