Keratin Acidic (AE1) anticorps

N° du produit ABIN187689

Cet anticorps anti-Keratin Acidic Monoclonal Souris (Clone AE-1) (ABIN187689) détecte spécifiquement Keratin Acidic dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Poulet, Boeuf (Vache) et Lapin.

Aperçu rapide pour Keratin Acidic (AE1) anticorps (ABIN187689)

Antigène: Keratin Acidic (AE1)

Reactivité: Humain, Rat, Souris, Poulet, Boeuf (Vache), Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AE-1

Détail du produit

Specificité: AE-1 detects in immunoblots distinct bands of 40 kDa, 45 kDa, 46 kDa, 48 kDa, 50 kDa, (50' kDa), 51 kDa, 55 kDa, and 56.5 kDa with all Ip's lower than 5.7. These MW's are believed to correspond with keratin 19, 18, 17, 16, 15, 14, 13, 12 and 10, respectively. Species Reactivity: Human, bovine, chicken, mouse, rabbit, and rat. Others not tested.

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry: (Formalin-fixed / Paraffin-embedded sections) Use at 1:150-1:200 for 30 min. at 37 °C. Higher dilutions can be used if sections are incubated at 4 °C overnight. Western blot: Use at 1:100. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Buffer: 250 µg purified IgG at 1 mg/ml with 0.1% sodium azide.

Stock: 4 °C

Détail du antigène

Antigène: Keratin Acidic (AE1)

Autre désignation: Cytokeratin (Epithelial type I)

Sujet: This antibody recognizes most of the acidic (type I) keratins. Each epithelium contains at least one acidic keratin. AE-1 is broadly reactive with almost all epithelial and their neoplasms. The antibody exhibits heterogenous staining within a given epithelium, the detailed staining pattern varies depending on the differentiated and/or growth state of the individual cells. This antibody together with MC-976 (clone AE-3) has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelial development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma.