H2AFX anticorps (N-Term)

N° du produit ABIN1882251

Cité dans 3 publications. Cet anticorps anti-H2AFX Monoclonal Souris (Clone 980CT1-5-5) (ABIN1882251) détecte spécifiquement H2AFX dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour H2AFX anticorps (N-Term) (ABIN1882251)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 980CT1-5-5

Détail du produit anti-H2AFX anticorps

Épitope: AA 1-30, N-Term

Homologie: Zf, M

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogène: This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:1000. IHC-P: 1:25

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 6 months

Références pour anticorps anti-H2AFX (ABIN1882251)

Stiff, ODriscoll, Rief, Iwabuchi, Löbrich, Jeggo: "ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation." dans: Cancer research, Vol. 64, Issue 7, pp. 2390-6, (2004) (PubMed).

Lukas, Melander, Stucki, Falck, Bekker-Jensen, Goldberg, Lerenthal, Jackson, Bartek, Lukas: "Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention." dans: The EMBO journal, Vol. 23, Issue 13, pp. 2674-83, (2004) (PubMed).

Park, Chan, Park, Oettinger, Kwon: "DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner." dans: Nucleic acids research, Vol. 31, Issue 23, pp. 6819-27, (2003) (PubMed).

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: H2AFX

Sujet: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Poids moléculaire: 15145

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus