H2AFX anticorps (C-Term)

N° du produit ABIN6971763

L’anticorps anti-H2AFX Polyclonal Lapin est utilisé pour la détection de H2AFX dans des échantillons de Humain. Il a été validé pour WB, IF, ICC et ChIP.

Aperçu rapide pour H2AFX anticorps (C-Term) (ABIN6971763)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-H2AFX anticorps

Épitope: C-Term

Attributs du produit: Histone H2AX (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. Histone H2A.X antibody (pAb) (H2AX) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogène: This Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2A.X

Poids moléculaire: 15 kDa

NCBI Accession: NP_002096

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus