GCLC anticorps (C-Term)

N° du produit ABIN190704

Cité dans 2 publications. Cet anticorps anti-GCLC Polyclonal Lapin (ABIN190704) détecte spécifiquement GCLC dans WB et IL. L’anticorps est réactif avec des échantillons de Zea mays, Tomato, Salicornia, Nicotiana tabacum et Arabidopsis thaliana.

Aperçu rapide pour GCLC anticorps (C-Term) (ABIN190704)

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivité: Zea mays, Tomato, Salicornia, Nicotiana tabacum, Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCLC est non-conjugé

Application: Western Blotting (WB), Immunolocalization (IL)

Détail du produit anti-GCLC anticorps

Épitope: C-Term

Fonction: Anti-Gamma-ECS | Gamma glutamylcysteine synthase

Specificité: The antibody recognizes closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)

Réactivité croisée (Details): Arabidopsis thaliana, Nicotiana tabcum, Salicornia sp., Solanum lycopersicum, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Marchantia polymorpha,Pisum sativum,Riciuns communis,Oryza sativa,Triticum aestivum

Purification: Protein G purified

Immunogène: KLH-conjugated synthetic peptide derived from C-terminal part of Zea mays gamma-ECS Q8W4W3

Isotype: IgG

Information d'application

Indications d'application: 1 : 5000 (IL), 1 : 5000 (WB)

Commentaires:

antibodies has been used in immunolocalization on Arabidopsis thaliana

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-GCLC (ABIN190704)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." dans: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Keefe, Hinz, Meins: "The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves." dans: Planta, Vol. 182, Issue 1, pp. 43-51, (2013) (PubMed).

Détails sur GCLC

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Autre désignation: Gamma-ECS

Sujet:

Background: Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Additional information: Total IgG concentration is 5,2 μg/ μl

Poids moléculaire: 50 kDa

ID gène: 828409

UniProt: Q8W4W3

Pathways: Cell RedoxHomeostasis