GCLC anticorps

N° du produit ABIN190704

Cet anticorps anti-GCLC est un anticorps Lapin Polyclonal détectant GCLC dans WB et IL. Adapté pour Zea mays, Tomato, Nicotiana tabacum et Arabidopsis thaliana. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour GCLC anticorps (ABIN190704)

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivité: Zea mays, Tomato, Nicotiana tabacum, Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCLC est non-conjugé

Application: Western Blotting (WB), Immunolocalization (IL)

Détail du produit anti-GCLC anticorps

Specificité: The antibody recognizes closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 50 kDa
This antibody can be used as a marker of vacuolar contents.

Purification: affinity purified

Immunogène: KLH-conjugated synthetic peptide derived from Zea mays gamma-ECS Q8W4W3

Isotype: IgG

Information d'application

Indications d'application: 1: 5000 with standard ECL (WB), 1: 5000 (IL)

Commentaires:

antibodies has been used in immunolocalization on Arabidopsis thaliana

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Références pour anticorps anti-GCLC (ABIN190704)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." dans: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Keefe, Hinz, Meins: "The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves." dans: Planta, Vol. 182, Issue 1, pp. 43-51, (2013) (PubMed).

Détails sur GCLC

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Autre désignation: Gamma-ECS

Sujet: AGI Code: At4g23100
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Poids moléculaire: 50 kDa

UniProt: Q8W4W3

Pathways: Cell RedoxHomeostasis