MICA anticorps (PE)

N° du produit ABIN192165

Cet anticorps anti-MICA est un anticorps Souris Monoclonal détectant MICA dans FACS. Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour MICA anticorps (PE) (ABIN192165)

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MICA est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: MEM-147

Détail du produit anti-MICA anticorps (PE)

Fonction: Anti-HLA Class I PE

Specificité: The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: PHA-activated peripheral blood lymphocytes

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-MICA (PE) (ABIN192165)

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." dans: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).

Détails sur MICA

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Autre désignation: HLA Class I

Sujet: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response