ELK1 anticorps (pSer389)

N° du produit ABIN196678

Cet anticorps Lapin Polyclonal détecte spécifiquement ELK1 dans IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour ELK1 anticorps (pSer389) (ABIN196678)

Antigène: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ELK1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ELK1 anticorps

Épitope: pSer389

Specificité: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Elk-1 (phospho-Ser389) antibody detects endogenous levels of Elk-1 only when phosphorylated at serine 389.

Purification: Immunoaffinity chromatography.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of serine 389 (P-R-SP-P-A).

Information d'application

Indications d'application: Suitable for use in Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur ELK1

Antigène: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Autre désignation: ELK1

Sujet: The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 (Ser383) is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.Synonyms: ETS domain-containing protein Elk-1, Elk-1

ID gène: 2002

NCBI Accession: NP_001107595

UniProt: P19419

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling