L’anticorps anti-STAT4 Polyclonal Lapin est utilisé pour la détection de STAT4 dans des échantillons de Humain et Souris. Il a été validé pour WB et IHC (p).
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. STAT4 (phospho-Tyr693) antibody detects endogenous levels of STAT4 only when phosphorylated at tyrosine 693.
Purification
Immunoaffinity chromatography.
Immunogène
The antiserum was produced against synthesized phosphopeptide derived from human STAT4 around the phosphorylation site of tyrosine 693 (K-G-YP-V-P).
Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody (in aliquots) at -20 °C.
Antigène
STAT4
(Signal Transducer and Activator of Transcription 4 (STAT4))
Autre désignation
STAT4
Sujet
Synonyms: Signal transducer and activator of transcription 4