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Pyruvate Kinase, Muscle (PKM) anticorps (Biotin)

Cet anticorps anti- est un anticorps Chèvre Polyclonal détectant dans WB et ELISA. Adapté pour Lapin.
N° du produit ABIN1968075

Aperçu rapide pour Pyruvate Kinase, Muscle (PKM) anticorps (Biotin) (ABIN1968075)

Antigène

Voir toutes Pyruvate Kinase, Muscle (PKM) Anticorps
Pyruvate Kinase, Muscle (PKM)

Reactivité

  • 28
  • 17
  • 11
  • 5
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
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Lapin

Hôte

  • 28
  • 2
  • 1
Chèvre

Clonalité

  • 30
  • 1
Polyclonal

Conjugué

  • 20
  • 3
  • 3
  • 2
  • 2
  • 1
Biotin

Application

  • 30
  • 17
  • 16
  • 11
  • 7
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Western Blotting (WB), ELISA
  • Specificité

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.

    Purification

    Ion exchange chromatography

    Immunogène

    Pyruvate Kinase [Rabbit Muscle].

    Type of Immunogen: Purified protein
  • Indications d'application

    Approved: ELISA (1:4000 - 1:20000), WB (1:500 - 1:2000)

    Usage: Anti-Pyruvate Kinase Antibody has been assayed against 1.0 μg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:15000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Commentaires

    Target Species of Antibody: Rabbit

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

    Conseil sur la manipulation

    avoid freeze thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
  • Antigène

    Pyruvate Kinase, Muscle (PKM)

    Autre désignation

    PKM / Pyruvate Kinase, Muscle

    Sujet

    Name/Gene ID: PKM

    Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

    ID gène

    5315

    Pathways

    L'effet Warburg
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