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Pyruvate Kinase, Muscle (PKM) anticorps (HRP)

Cet anticorps anti- est un anticorps Chèvre Polyclonal détectant dans WB et ELISA. Adapté pour Lapin.
N° du produit ABIN1968087

Aperçu rapide pour Pyruvate Kinase, Muscle (PKM) anticorps (HRP) (ABIN1968087)

Antigène

Voir toutes Pyruvate Kinase, Muscle (PKM) Anticorps
Pyruvate Kinase, Muscle (PKM)

Reactivité

  • 28
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  • 5
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  • 2
  • 2
  • 1
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Lapin

Hôte

  • 28
  • 2
  • 1
Chèvre

Clonalité

  • 30
  • 1
Polyclonal

Conjugué

  • 20
  • 3
  • 3
  • 2
  • 2
  • 1
HRP

Application

  • 30
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Western Blotting (WB), ELISA
  • Specificité

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.

    Purification

    Ion exchange chromatography

    Immunogène

    Pyruvate Kinase [Rabbit Muscle].

    Type of Immunogen: Purified protein
  • Indications d'application

    Approved: ELISA (1:4000 - 1:20000), WB (1:500 - 1:2000)

    Usage: Anti-Pyruvate Kinase has been assayed against 1.0 μg of Pyruvate Kinase [Rabbit Muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:15000 to 1:60000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Commentaires

    Target Species of Antibody: Rabbit

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

    Conseil sur la manipulation

    avoid freeze thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
  • Antigène

    Pyruvate Kinase, Muscle (PKM)

    Autre désignation

    PKM / Pyruvate Kinase, Muscle

    Sujet

    Name/Gene ID: PKM

    Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

    ID gène

    5315

    Pathways

    L'effet Warburg
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