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CDC25A anticorps (Ser75)

Cet anticorps anti-CDC25A est un anticorps Lapin Polyclonal détectant CDC25A dans WB. Adapté pour Humain et Souris.
N° du produit ABIN197370

Aperçu rapide pour CDC25A anticorps (Ser75) (ABIN197370)

Antigène

Voir toutes CDC25A Anticorps
CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivité

  • 199
  • 68
  • 53
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Humain, Souris

Hôte

  • 208
  • 7
Lapin

Clonalité

  • 209
  • 6
Polyclonal

Conjugué

  • 96
  • 17
  • 14
  • 13
  • 9
  • 9
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
Cet anticorp CDC25A est non-conjugé

Application

  • 173
  • 103
  • 57
  • 40
  • 40
  • 29
  • 19
  • 17
  • 15
  • 8
  • 7
  • 6
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Épitope

    • 32
    • 27
    • 18
    • 17
    • 15
    • 13
    • 9
    • 9
    • 8
    • 8
    • 7
    • 7
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser75

    Specificité

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. cdc25A antibody AP02684PU detects endogenous levels of total cdc25A protein.

    Purification

    Immunoaffinity chromatography.

    Immunogène

    The antiserum was produced against synthesized non-phosphopeptide derived from human cdc25A around the phosphorylation site of serine 75 (M-G-Sp-S-E).
  • Indications d'application

    Suitable for use in Western blot (1: 500-1: 1000).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Store the antibody (in aliquots) at -20 °C.
  • Antigène

    CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

    Autre désignation

    CDC25A

    Sujet

    Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

    ID gène

    993

    NCBI Accession

    NP_001780

    UniProt

    P30304

    Pathways

    Cycle Cellulaire, Mitotic G1-G1/S Phases, M Phase
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