CDC25A anticorps (Ser75)
Aperçu rapide pour CDC25A anticorps (Ser75) (ABIN197370)
Antigène
Voir toutes CDC25A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- Ser75
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Specificité
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. cdc25A antibody AP02684PU detects endogenous levels of total cdc25A protein.
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Purification
- Immunoaffinity chromatography.
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Immunogène
- The antiserum was produced against synthesized non-phosphopeptide derived from human cdc25A around the phosphorylation site of serine 75 (M-G-Sp-S-E).
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Indications d'application
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Suitable for use in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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Autre désignation
- CDC25A
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Sujet
- Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1
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ID gène
- 993
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NCBI Accession
- NP_001780
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UniProt
- P30304
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Pathways
- Cycle Cellulaire, Mitotic G1-G1/S Phases, M Phase
Antigène
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