MSK1 anticorps (Ser376)

N° du produit ABIN197422

Détail du produit anti-MSK1 anticorps

Antigène: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Épitope: Ser376

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MSK1 est non-conjugé

Application: Western Blotting (WB)

Specificité: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. MSK1 antibody AP02710PU detects endogenous levels of total MSK1 protein.

Purification: Immunoaffinity chromatography.

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from human MSK1 around the phosphorylation site of serine 376 (G-Y-Sp-F-V).

Information d'application

Indications d'application: Suitable for use in in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur MSK1

Antigène: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Autre désignation: RPS6KA5 / MSK1 (RPS6KA5 Produits)

Synonymes: anticorps MSK1, anticorps MSPK1, anticorps RLPK, anticorps 3110005L17Rik, anticorps 6330404E13Rik, anticorps AI854034, anticorps Msk1, anticorps ribosomal protein S6 kinase A5, anticorps ribosomal protein S6 kinase, polypeptide 5, anticorps RPS6KA5, anticorps Rps6ka5

Sujet: MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain.Synonyms: 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKL, Ribosomal protein S6 kinase alpha-5

ID gène: 9252

NCBI Accession: NP_004746

UniProt: O75582

Pathways: Signalisation MAPK, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades