Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
This antibody detects endogenous levels of total p70 S6 Kinase protein.
Purification
Affinity chromatography
Immunogène
The antiserum was produced against synthesized non-phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 424 (P-V-Sp-P-V).
RPS6KB1
Reactivité: Humain, Souris, Rat, Porc
WB, IHC, ELISA, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody (in aliquots) at -20 °C.
Antigène
RPS6KB1
(Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))