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Sonic Hedgehog anticorps (AA 199-437)

Cet anticorps anti-Sonic Hedgehog est un anticorps Rat Monoclonal détectant Sonic Hedgehog dans WB et ELISA. Adapté pour Souris.
N° du produit ABIN6757522

Aperçu rapide pour Sonic Hedgehog anticorps (AA 199-437) (ABIN6757522)

Antigène

Voir toutes Sonic Hedgehog (SHH) Anticorps
Sonic Hedgehog (SHH)

Reactivité

  • 73
  • 54
  • 38
  • 17
  • 10
  • 6
  • 6
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Souris

Hôte

  • 79
  • 16
  • 8
  • 1
  • 1
Rat

Clonalité

  • 71
  • 34
Monoclonal

Conjugué

  • 60
  • 10
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Sonic Hedgehog est non-conjugé

Application

  • 78
  • 33
  • 28
  • 14
  • 14
  • 13
  • 12
  • 11
  • 10
  • 10
  • 5
  • 4
  • 2
  • 1
Western Blotting (WB), ELISA
  • Épitope

    • 15
    • 12
    • 9
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 199-437

    Specificité

    Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not cross-react with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide.

    Purification

    Protein G purified

    Immunogène

    Purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide, aa 199-437).

    Isotype

    IgG2a
  • Indications d'application

    Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot and ELISA. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for rmShh C-terminal peptide is approximately 1 ng/well. Western Blot: 1-2 μg/mL with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for recombinant mouse Shh C-terminal peptide is approximately 0.5 ng/lane under non-reducing and reducing conditions.

    Commentaires

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.2, 5 % trehalose.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Antigène

    Sonic Hedgehog (SHH)

    Autre désignation

    Sonic Hedgehog / SHH

    Sujet

    Name/Gene ID: SHH
    Subfamily: Cysteine C46
    Family: Protease

    Synonyms: SHH, HHG-1, HLP3, Sonic hedgehog, Sonic hedgehog homolog, MCOPCB5, Sonic hedgehog protein, HHG1, HPE3, SMMCI, TPT, TPTPS

    ID gène

    6469

    UniProt

    Q15465

    Pathways

    Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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