VASP anticorps (pSer239)

N° du produit ABIN198238

Cet anticorps Souris Monoclonal détecte spécifiquement VASP dans WB, ELISA, IP, ICC et FACS. Il présente une réactivité envers Humain, Souris, Rat, Singe, Chien, Boeuf (Vache) et Cheval.

Aperçu rapide pour VASP anticorps (pSer239) (ABIN198238)

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivité: Humain, Souris, Rat, Singe, Chien, Boeuf (Vache), Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VASP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 16C2

Détail du produit anti-VASP anticorps

Épitope: AA 236-242, pSer239

Specificité: Recognizes human phosphorylated VASP, Mr 50kD. Species cross-reactivity: mouse.

Purification: Gel filtration

Immunogène: KLH-conjugated, synthetic phosphopeptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA (0.05 μg/mL), Flo (2.4 μg/mL), ICC, IP, WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry, FACS and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3 μg/mL antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, fixed with 4 % paraformaldehyde and permeabilized with 0.2 % Triton-X. FACS: 2.4 μg/mL using fixed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. ELISA: 0.05 μg/mL, as reported by an independent laboratory.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL ddH2O

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder from PBS, 0.09 % sodium azide, PEG, sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur VASP

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Autre désignation: VASP

Sujet: Name/Gene ID: VASP
Family: Ena-VASP

Synonyms: VASP

ID gène: 7408

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation