Cyclin D1 anticorps

N° du produit ABIN200701

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin D1 dans WB, IHC, ELISA, FACS, IP, IHC (p) et IHC (fro). Il présente une réactivité avec des échantillons de Humain et Rat.

Aperçu rapide pour Cyclin D1 anticorps (ABIN200701)

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Cyclin D1 anticorps

Specificité: Recognizes the Cyclin D1 (36kD). Species cross-reactivity: Human and rat.

Purification: Protein A purified

Immunogène: Human cyclin D1.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P, IP, WB (1:2000)

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), ELISA, Immunoprecipitation and Western Blot. Western Blot: 1:2000. Flow Cytometry: Neat, Use 5 μL of the optimal working dilution to stain 1 x10⁶ in 100 μL. Permeabilization is recommended prior to staining. Immunohistochemistry (Paraffin and frozen): 1:1000-1:10000. Note: Paraffin sections require antigen retrieval using sodium citrate buffer,  pH 6.0, heat treatment prior to staining. Positive control: Small intestine, colon.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Autre désignation: CCND1 / Cyclin D1

Sujet: Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene

ID gène: 595

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling