EPH Receptor A2 anticorps (Extracellular Domain)
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- Antigène Voir toutes EPH Receptor A2 (EPHA2) Anticorps
- EPH Receptor A2 (EPHA2)
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Épitope
- Extracellular Domain
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Reactivité
- Humain, Souris, Chien, Boeuf (Vache)
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp EPH Receptor A2 est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificité
- Recognizes human EphA2.
- Purification
- Protein G purified
- Immunogène
- NS0-derived rhEphA2 extracellular domain.
- Isotype
- IgG2a
- Top Product
- Discover our top product EPHA2 Anticorps primaire
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- Indications d'application
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Approved: Flo (25 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 μg/mL. Add 10 μL to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 μg/mL to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 200 μL sterile PBS.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.4, 5 % trehalose
- Conseil sur la manipulation
- Avoid frequent freeze thaws. Store undiluted.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Store at -20°C to -70°C. Stable for 1 year from date of receipt.
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- Antigène
- EPH Receptor A2 (EPHA2)
- Autre désignation
- EPHA2 / EPH Receptor A2 (EPHA2 Produits)
- Synonymes
- anticorps ARCC2, anticorps CTPA, anticorps CTPP1, anticorps CTRCT6, anticorps ECK, anticorps epha2, anticorps MGC89505, anticorps AW545284, anticorps Eck, anticorps Myk2, anticorps Sek-2, anticorps Sek2, anticorps id:ibd5152, anticorps rtk6, anticorps G42, anticorps XE10, anticorps epha2-A, anticorps EPH receptor A2, anticorps Eph receptor A2, anticorps epoxide hydrolase, anticorps eph receptor A2 a, anticorps EPH receptor A2 L homeolog, anticorps EPHA2, anticorps Epha2, anticorps epha2, anticorps ephA2, anticorps epha2a, anticorps epha2.L
- Sujet
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Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase
Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1 - ID gène
- 1969
- UniProt
- P29317
- Pathways
- Signalisation RTK
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