MMP14 anticorps (AA 319-333)

N° du produit ABIN209030

Détail du produit anti-MMP14 anticorps

Antigène: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Épitope: AA 319-333

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe, Chévre, Hamster, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MMP14 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).

Purification: Immunoaffinity purified

Immunogène: Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).

Isotype: IgG3 kappa

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 2 % BSA (protease free).

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur MMP14

Antigène: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Autre désignation: MMP14 / MMP-14 (MMP14 Produits)

Synonymes: anticorps MMP1, anticorps MMP-14, anticorps MMP-X1, anticorps MT-MMP, anticorps MT-MMP 1, anticorps MT1-MMP, anticorps MT1MMP, anticorps MTMMP1, anticorps mmp-x1, anticorps mt1-mmp, anticorps mtmmp1, anticorps AI325305, anticorps MT-MMP-1, anticorps sabe, anticorps Mt1-mmp, anticorps MT-MMPbeta, anticorps cb386, anticorps sb:cb386, anticorps wu:fb72b09, anticorps mmp14, anticorps matrix metallopeptidase 14, anticorps matrix metallopeptidase 14 (membrane-inserted), anticorps matrix metallopeptidase 14b (membrane-inserted), anticorps matrix metallopeptidase 14a (membrane-inserted), anticorps matrix metallopeptidase 14 L homeolog, anticorps MMP14, anticorps mmp14, anticorps Mmp14, anticorps mmp14b, anticorps mmp14a, anticorps mmp14.L

Sujet: Name/Gene ID: MMP14
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1

ID gène: 4323

UniProt: P50281

Pathways: Autophagy