Caspase 3 anticorps

N° du produit ABIN210737

L’anticorps anti-Caspase 3 Polyclonal Lapin est utilisé pour la détection de Caspase 3 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB.

Aperçu rapide pour Caspase 3 anticorps (ABIN210737)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Caspase 3 anticorps

Specificité: Recognizes full-length Caspase 3 (Yama/Apopain) at 32kD and proteolytic fragments as demonstrated after in vitro cleavage of recombinant Caspase 3. Cross-reacts with human, mouse and rat.

Purification: Protein A purified

Immunogène: Human full-length Caspase 3 fusion protein containing a histidine-6 tag.

Type of Immunogen: Fusion protein

Isotype: IgG

Information d'application

Indications d'application: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.5-2 μg/mL (will detect the 32kD precursor of Caspase 3 in RIPA lysates from non-stimulated human A431 cells, previous lots detected Caspase 3 in RIPA lysates of mouse 3T3 and rat PC12 cells). Use 20 μg per lane for minigels. Positive Antigen Control: non-stimulated A431 cell lysate. Western Blot Analysis: Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 3 (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.07 M Tris-glycine,  pH 7.4, 0.105 M sodium chloride, 0.035 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: CASP3 / Caspase 3

Sujet: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

ID gène: 836

UniProt: P42574

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation