Nibrin anticorps (AA 420-774)

N° du produit ABIN210930

Cet anticorps anti-Nibrin est un anticorps Souris Monoclonal détectant Nibrin dans WB et IP. Adapté pour Humain.

Aperçu rapide pour Nibrin anticorps (AA 420-774) (ABIN210930)

Antigène: Nibrin (NBN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nibrin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-Nibrin anticorps

Épitope: AA 420-774

Specificité: Recognizes human Nibrin. Recognizes Nibrin/Nbs1, Mr 95kD. A non-specific protein was also detected at Mr 33kD in some lysates, but not Jurkat cell lysate. Species cross-reactivity: Human, NOT mouse.

Purification: Protein G purified

Immunogène: GST fusion protein corresponding to residues 420-774 of human Nibrin.

Type of Immunogen: Fusion protein

Isotype: IgG

Information d'application

Indications d'application: Approved: IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Nibrin/Nbs1 in Jurkat, HeLa nuclear extract, HeLa whole cell, and A431+/-EGF lysates. Included Positive Antigen Control: Jurkat lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Immunoprecipitation: 4 μg immunoprecipitates Nibrin/Nbs1 from 500 μg of Jurkat RIPA lysate. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Nibrin/Nbs1 (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Nibrin/Nbs1 (95kD).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur Nibrin

Antigène: Nibrin (NBN)

Autre désignation: NBN / NBS1 / Nibrin

Sujet: Name/Gene ID: NBN

Synonyms: NBN, ATV, AT-V2, Nibrin, NBS1, AT-V1, NBS, p95

ID gène: 4683

UniProt: O60934

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response