Calreticulin anticorps (C-Term)

N° du produit ABIN211075

Cet anticorps Lapin Polyclonal détecte spécifiquement Calreticulin dans WB et IHC. Il présente une réactivité envers Humain, Souris, Rat, Porc, Boeuf (Vache) et Xenopus laevis.

Aperçu rapide pour Calreticulin anticorps (C-Term) (ABIN211075)

Antigène: Calreticulin (CALR)

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calreticulin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Calreticulin anticorps

Épitope: AA 412-417, C-Term

Specificité: Specific for calreticulin. Does not cross-react with other endoplasmic reticulum proteins. Species cross-reactivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.

Purification: Protein A purified

Immunogène: Synthetic peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin, KLH conjugated.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, WB (0.5 - 2 μg/mL)

Usage: Suitable for Western Blot and Immunohistochemistry. Western Blot: 0.5-2 μg/mL detects calreticulin in RIPA lysates from murine 3T3/NIH fibroblasts and human foreskin fibroblast (HFF). 20 μg 3T3/NIH and 10 μg HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-human Calreticulin (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects calreticulin in tissue sections and cells. Included Positive Antigen Control: 3T3 lysate. Use 20 μg per lane for minigels.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Frozen solution in 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Calreticulin

Antigène: Calreticulin (CALR)

Autre désignation: CALR / Calreticulin

Sujet: Name/Gene ID: CALR

Synonyms: CALR, Calregulin, CC1qR, CRP55, CRT, CRTC, ERp60, Grp60, RO, SSA, Calreticulin, HACBP

ID gène: 811

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response