Calreticulin anticorps (C-Term)

N° du produit ABIN211075

Détail du produit anti-Calreticulin anticorps

Antigène: Calreticulin (CALR)

Épitope: AA 412-417, C-Term

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calreticulin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificité: Specific for calreticulin. Does not cross-react with other endoplasmic reticulum proteins. Species cross-reactivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.

Purification: Protein A purified

Immunogène: Synthetic peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin, KLH conjugated.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, WB (0.5 - 2 μg/mL)

Usage: Suitable for Western Blot and Immunohistochemistry. Western Blot: 0.5-2 μg/mL detects calreticulin in RIPA lysates from murine 3T3/NIH fibroblasts and human foreskin fibroblast (HFF). 20 μg 3T3/NIH and 10 μg HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-human Calreticulin (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects calreticulin in tissue sections and cells. Included Positive Antigen Control: 3T3 lysate. Use 20 μg per lane for minigels.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Frozen solution in 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Calreticulin

Antigène: Calreticulin (CALR)

Autre désignation: CALR / Calreticulin (CALR Produits)

Synonymes: anticorps CRT, anticorps RO, anticorps SSA, anticorps cC1qR, anticorps crc, anticorps crt, anticorps Calregulin, anticorps CG9429, anticorps Clr, anticorps Dmel\\CG9429, anticorps anon-EST:Liang-2.11, anticorps anon-EST:Liang-2.17, anticorps anon-EST:fe3D7, anticorps clone 2.11, anticorps clone 2.17, anticorps eth, anticorps potp, anticorps cc1qr, anticorps ssa, anticorps NV19007, anticorps CRP55, anticorps ERp60, anticorps HACBP, anticorps CHOT1, anticorps CHT1, anticorps CT1, anticorps AA589632, anticorps CRTR, anticorps Creat, anticorps CCDS1, anticorps calrl2, anticorps fc66d10, anticorps wu:fc66d10, anticorps wu:fj50c03, anticorps zgc:66153, anticorps calreticulin, anticorps calreticulin L homeolog, anticorps Calreticulin, anticorps putative calreticulin, anticorps solute carrier family 6 member 8, anticorps solute carrier family 6 (neurotransmitter transporter, creatine), member 8, anticorps CALR, anticorps calr.L, anticorps Crt, anticorps crt, anticorps CRT, anticorps Calr, anticorps calr, anticorps LMJF_31_2600, anticorps Crc, anticorps Slc6a8, anticorps SLC6A8, anticorps LOC8269812

Sujet: Name/Gene ID: CALR

Synonyms: CALR, Calregulin, CC1qR, CRP55, CRT, CRTC, ERp60, Grp60, RO, SSA, Calreticulin, HACBP

ID gène: 811

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response