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Leptin anticorps (AA 22-167)

Cet anticorps Souris Monoclonal détecte spécifiquement Leptin dans WB, IHC, IP et ICC. Il présente une réactivité envers Humain.
N° du produit ABIN2117865

Aperçu rapide pour Leptin anticorps (AA 22-167) (ABIN2117865)

Antigène

Voir toutes Leptin (LEP) Anticorps
Leptin (LEP)

Reactivité

  • 131
  • 77
  • 71
  • 12
  • 8
  • 8
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
Humain

Hôte

  • 180
  • 37
  • 7
  • 5
  • 2
  • 2
  • 1
Souris

Clonalité

  • 193
  • 40
Monoclonal

Conjugué

  • 123
  • 27
  • 14
  • 9
  • 7
  • 7
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Leptin est non-conjugé

Application

  • 146
  • 82
  • 80
  • 48
  • 39
  • 39
  • 36
  • 35
  • 30
  • 18
  • 14
  • 11
  • 10
  • 7
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone

D4
  • Épitope

    • 28
    • 16
    • 15
    • 15
    • 9
    • 9
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 22-167

    Fonction

    Monoclonal Antibody to Leptin (LEP)

    Séquence

    MGHHHHHHSG S-VPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC

    Specificité

    The antibody is a mouse monoclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.

     Réactivité croisée

    Porc

    Purification

    Protein A + Protein G affinity chromatography

    Immunogène

    RPA084Hu01Recombinant Leptin (LEP)

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    Leptin (LEP)

    Autre désignation

    Leptin

    Sujet

    OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein

    Pathways

    Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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