IGF1 anticorps

N° du produit ABIN211952

L’anticorps anti-IGF1 Monoclonal produit chez le Souris a été validé pour WB, IHC, IP, Neut et IHC (p). Il convient pour la détection de IGF1 dans des échantillons provenant de Humain, Souris et Rat. }

Aperçu rapide pour IGF1 anticorps (ABIN211952)

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IGF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-IGF1 anticorps

Specificité: Recognizes human IGF1. Cross-reactivity with IGF-II: ~40 % . Species cross-reactivity: mouse, rat and possibly chicken. Occasional difficulty neutralizing rat IGF1 due to binding protein interference.

Purification: Protein G purified

Immunogène: Human insulin-like growth factor I (IGF1) purified from human plasma.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), IP, Neut (10 - 20 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Neutralization and Immunohistochemistry. Immunoprecipitation: 5 μg immunoprecipitated 100 ng IGF1. Neutralization: 10-20 μg/mL can inhibit the activity of 10 ng/mL of IGF1 as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay. Immunohistochemistry: 10 μg/mL. Reported to detect IFG1 in formalin-fixed, paraffin-embedded skin sections.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 200 μL sterile ddH2O

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder from 0.1M Tris-glycine,  pH 7.4, 0.15  mM sodium chloride, 0.1  mM EDTA. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid frequent freeze and thaws.

Stock: -20 °C

Stockage commentaire: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Détails sur IGF1

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: IGF-I / IGF1

Sujet: Name/Gene ID: IGF1
Family: Hormone

Synonyms: IGF1, IGF-IA, IGF-IB, IGFI, IBP1, IGF-I, Insulin-like growth factor 1, Insulin-like growth factor I, Insulin-like growth factor IA, Insulin-like growth factor IB, Mechano growth factor, Somatomedin-C, IGF1A, MGF

ID gène: 3479

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation