SIRT1 anticorps (AA 207-256)

N° du produit ABIN214144

Cet anticorps Lapin Polyclonal détecte spécifiquement SIRT1 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Chien, Lapin, Singe, Boeuf (Vache), Cobaye, Cheval, Poisson zèbre (Danio rerio), Roussette (Chauve-souris) et Porc.

Aperçu rapide pour SIRT1 anticorps (AA 207-256) (ABIN214144)

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Reactivité: Humain, Souris, Rat, Chien, Lapin, Singe, Boeuf (Vache), Cobaye, Cheval, Poisson zèbre (Danio rerio), Roussette (Chauve-souris), Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIRT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SIRT1 anticorps

Épitope: AA 207-256

Marque: IHC-plus™

Specificité: Human SIRT1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Xenopus (92%) Chicken (85%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa207-256 of human SIRT1 (Q96EB6, NP_036370). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Turkey, Xenopus (92%), Chicken (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur SIRT1

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Autre désignation: SIRT1 / Sirtuin 1

Sujet: Name/Gene ID: SIRT1
Family: ADP-Ribosyltransferase

Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1

ID gène: 23411

NCBI Accession: NP_036370

UniProt: Q96EB6

Pathways: Signalisation MAPK, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling