CD3 anticorps (FITC)

N° du produit ABIN2144454

Cet anticorps anti-CD3 est un anticorps Souris Monoclonal détectant CD3 dans FACS, IHC, Func, IF, WB et ICC. Adapté pour Humain.

Aperçu rapide pour CD3 anticorps (FITC) (ABIN2144454)

Antigène: CD3

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC)

Clone: 4AOKT3

Détail du produit anti-CD3 anticorps (FITC)

Attributs du produit: 4AOKT3 reacts with CD3, a 20-26 kDa molecule, which is expressed on all mature T lymphocytes (approximately 60-80 % of normal human peripheral blood lymphocytes), NK-T cells and some thymocytes. CD3 associated with the T-cell receptor a/b or g/d dimer also plays a role in T-cell activation and signal transduction during antigen recognition.* CD3 mAb is used to count mature T cells in blood.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway