CD21 anticorps (FITC)
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- Antigène Voir toutes CD21 (CR2) Anticorps
- CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD21 est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Attributs du produit
- 4ABU32 reacts with CD21 protein, a 145 kDa receptor for C3d complement fragment and for Epstein-Barr virus (EBV), expressed on all mature B lymphocytes from sIg+ stage, then lost upon activation. It also present on follicular dendritic cells and some epithelia cells. It is reported CD21 weakly expressed on the subset of mature T -cells. CD21 plays a role in B cell activation and proliferation and is part of a large signal-transduction complex containing CD19, CD81and other molecules as regulator of complement activation.This clone is cross reactive with non-human primate .*CD21 is a phenotyping marker of lymphoma.
- Clone
- 4ABU32
- Isotype
- IgG1
- Top Product
- Discover our top product CR2 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))
- Autre désignation
- CD21 (CR2 Produits)
- Synonymes
- anticorps C3DR, anticorps CD21, anticorps CR, anticorps CVID7, anticorps SLEB9, anticorps Cd21, anticorps cd21, anticorps CR1, anticorps CR1-L, anticorps CR2, anticorps complement C3d receptor 2, anticorps complement C3b/C4b receptor 1 (Knops blood group), anticorps CR2, anticorps Cr2, anticorps CR1
- Pathways
- Système du Complément
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