CD47 anticorps (PE)

N° du produit ABIN2144904

Cet anticorps anti-CD47 est un anticorps Souris Monoclonal détectant CD47 dans FACS. Adapté pour Humain.

Aperçu rapide pour CD47 anticorps (PE) (ABIN2144904)

Antigène: CD47

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: 4ACC2C6D4

Détail du produit anti-CD47 anticorps (PE)

Attributs du produit: 4ACC2C6D4 reacts with CD47 ( gp42 ), a 45-55 kDa molecule, expressed on broad tissue and cells including hemopoietic cells , epithelial, endothelial cells and other tissue cells. CD47 antigen function on adhesion molecule and thrombospondin receptor.This clone is cross reactive with non-human primate.

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur CD47

Antigène: CD47

Autre désignation: CD47