CDK1 anticorps

N° du produit ABIN215066

L’anticorps Souris Monoclonal anti-CDK1 a été validé pour WB, IHC, IF, IP, IHC (p), IHC (fro) et Func. Il convient pour détecter CDK1 dans des échantillons de Humain, Souris, Rat, Poulet et Xenopus laevis.

Aperçu rapide pour CDK1 anticorps (ABIN215066)

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivité: Humain, Souris, Rat, Poulet, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Détail du produit anti-CDK1 anticorps

Specificité: Recognizes a 34kD protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks). Human, mouse, rat, chicken, Xenopus.

Purification: Purified

Immunogène: Xenopus p34cdc2.

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Func, IF, IHC, IHC-Fr, IHC-P, IP, WB (2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Immunoprecipitation (Native, denatured): 2 μg/mg protein lysate using Protein A. Immunohistochemistry (formalin/paraffin, frozen): 2-4 μg/mL for 30 minutes at RT. Paraffin sections require antigen retrieval. Western Blot: 2 μg/mL for 2 hours at RT. Positive control: HeLa or NIH3T3 cells, germinal center in a tonsil or reactive lymph node.

Commentaires:

Target Species of Antibody: Xenopus

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur CDK1

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Autre désignation: CDK1 / CDC2

Sujet: Name/Gene ID: CDK1
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

ID gène: 983

UniProt: P06493

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA