CDKN1B anticorps (pSer10)

N° du produit ABIN215394

Cet anticorps anti-CDKN1B est un anticorps Lapin Polyclonal détectant CDKN1B dans WB, IF et IP. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour CDKN1B anticorps (pSer10) (ABIN215394)

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDKN1B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-CDKN1B anticorps

Épitope: pSer10

Specificité: Recognizes p27 when it is phosphorylated at the Ser10 residue. Lysates of HEK 293T cells transfected with an expression plasmid encoding a p27 mutant where Ser10 was substituted with an alanine residue were used to confirm the antibody's specificity. Reactivity was confirmed with HEK293 cells expressing wild-type p27/KIP that is normally phosphorylated. NIH3T3 and MCF-7 cell lysates can also be used as positive controls. Species cross-reactivity: Human, rat and mouse.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IF (5 - 10 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1-3 μg/mL. Immunoprecipitation: 5-10 μg/IP reaction. Immunofluorescence: 5-10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur CDKN1B

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: CDKN1B / p27 Kip1

Sujet: Name/Gene ID: CDKN1B

Synonyms: CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4

ID gène: 1027

UniProt: P46527

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy