C1S anticorps

N° du produit ABIN2191986

Cet anticorps anti-C1S est un anticorps Souris Monoclonal détectant C1S dans WB, FACS, IP, IHC (p), IHC (fro), IA et InhA. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour C1S anticorps (ABIN2191986)

Antigène: C1S (Complement Component 1, S Subcomponent (C1S))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp C1S est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA), Inhibition Assay (InhA)

Clone: M81

Détail du produit anti-C1S anticorps

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of C4 activation by C1s dilutions have to be made according to the amounts C1s to be inactivated.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-C1S (ABIN2191986)

Matsumoto, Nagaki, Kitamura, Kuramitsu, Nagasawa, Seya: "Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2743-50, (1989) (PubMed).

Détails sur C1S

Antigène: C1S (Complement Component 1, S Subcomponent (C1S))

Autre désignation: c1s

Sujet: Monoclonal antibody M81 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen, MW 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M81 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M81 blocks C4 activation and C4 binding to activated C1s. The antibody reacts around the binding site of C1s and reacts with both active and inactive C1s.

Pathways: Système du Complément