C1S anticorps (Complement Component 1, S Subcomponent)

Details for Product anti-C1S Antibody No. ABIN2191986, Fournisseur: Connectez-vous pour afficher
Antigène
  • AA959438
  • AI255193
  • AI327365
  • C1sa
  • r-gsp
  • C1S
  • complement C1s
  • complement component 1, s subcomponent 1
  • complement component 1, s subcomponent
  • complement component 1, s subcomponent L homeolog
  • complement C1s subcomponent
  • C1S
  • C1s1
  • C1s
  • c1s
  • c1s.L
  • LOC100558615
Reactivité
Humain
135
25
25
5
2
Hôte
Souris
72
59
4
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp C1S est non-conjugé
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Inhibition Assay (InhA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
72
60
38
16
15
15
13
8
6
3
3
3
2
1
1
1
1
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Clone M81
Stérilité 0.2 μm filtered
Plasmids, Primers & others Plasmids, Primers & others C1S products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation c1s (C1S Antibody Extrait)
Sujet Monoclonal antibody M81 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen, MW 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M81 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M81 blocks C4 activation and C4 binding to activated C1s. The antibody reacts around the binding site of C1s and reacts with both active and inactive C1s.
Pathways Système du Complément
Indications d'application For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of C4 activation by C1s dilutions have to be made according to the amounts C1s to be inactivated.
Restrictions For Research Use only
Buffer PBS containing 0.1 % bovine serum albumin.
Stock 4 °C
Stockage commentaire Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Date de péremption 12 months
Produit citée dans: Matsumoto, Nagaki, Kitamura, Kuramitsu, Nagasawa, Seya: "Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2743-50, 1989 (PubMed).

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