Caspase 3 anticorps (N-Term)

N° du produit ABIN219240

Cet anticorps anti-Caspase 3 est un anticorps Lapin Polyclonal détectant Caspase 3 dans WB. Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Porc, Cobaye, Mouton, Poulet et Saccharomyces cerevisiae.

Aperçu rapide pour Caspase 3 anticorps (N-Term) (ABIN219240)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Porc, Cobaye, Mouton, Poulet, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Caspase 3 anticorps

Épitope: N-Term

Specificité: Recognizes human caspase-3. In apoptotic cells, this antibody also detects 20kD and 18kD proteins which correspond to the processed forms of caspase-3. Following the induction of apoptosis and the appearance of these smaller bands, a concurrent reduction in the amount of the procaspase enzyme is noted on immunoblot analysis. A weak ~30kD band may be detected in some samples. This band is not caspase-3 as it is detected in MCF7 cell lysates which lack the caspase-3 protein. Species cross-reactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit and yeast.

Purification: Protein A purified

Immunogène: Recombinant full length human caspase-3 protein with an N-terminal (His) 6 tag.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: WB (1 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot (ECL): 1 μg/mL. A band is seen at ~36kD. Positive control: HeLa Nuclear Extract.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: CASP3 / Caspase 3

Sujet: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

ID gène: 836

UniProt: P42574

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation