ENOS anticorps (pSer1179)

N° du produit ABIN224813

L’anticorps anti-ENOS Polyclonal Lapin est utilisé pour la détection de ENOS dans des échantillons de Humain, Rat, Souris, Porc, Boeuf (Vache), Chien et Cobaye. Il a été validé pour WB, ELISA et IF.

Aperçu rapide pour ENOS anticorps (pSer1179) (ABIN224813)

Antigène: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivité: Humain, Rat, Souris, Porc, Boeuf (Vache), Chien, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ENOS est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-ENOS anticorps

Épitope: pSer1179

Specificité: Recognizes bovine eNOS protein, phosphorylated at Ser1179 (~135kD). Reactivity has been confirmed with VEGF-stimulated, serum-starved BAEC (bovine aortic endothelial cell) lysates. A very low signal was observed in the lysates of serum-starved, unstimulated BAEC and no signal was observed in lysates of unstimulated BAEC in the presence of serum. Species sequence homology: human, rat, mouse, canine, porcine, and guinea pig.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide derived from the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), IF, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA and Western Blot. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunofluorescence: reactive with monkey COS cells in immunofluorescence assays (per Fulton D, et al. 2002 publication.).

Commentaires:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur ENOS

Antigène: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Autre désignation: NOS3 / eNOS

Sujet: Name/Gene ID: NOS3

Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3

ID gène: 4846

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling