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CENPA anticorps (AA 4-17)

Cet anticorps anti-CENPA est un anticorps Lapin Polyclonal détectant CENPA dans WB. Adapté pour Humain et Singe.
N° du produit ABIN232755

Aperçu rapide pour CENPA anticorps (AA 4-17) (ABIN232755)

Antigène

Voir toutes CENPA Anticorps
CENPA (Centromere Protein A (CENPA))

Reactivité

  • 68
  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
Humain, Singe

Hôte

  • 54
  • 14
Lapin

Clonalité

  • 53
  • 15
Polyclonal

Conjugué

  • 30
  • 5
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CENPA est non-conjugé

Application

  • 51
  • 25
  • 16
  • 13
  • 13
  • 12
  • 10
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Épitope

    • 16
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 4-17

    Specificité

    Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.

    Homologie

    Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Rabbit (93%) Nectri (86%).

    Purification

    Immunoaffinity purified

    Immunogène

    Synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A. KLH conjugated. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Rabbit (93%), Nectri (86%).

    Type of Immunogen: Synthetic peptide - KLH conjugated

    Isotype

    IgG
  • Indications d'application

    Approved: WB

    Usage: Suitable for use in Western Blot analysis. Western Blot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20 μg control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2 μg/mL anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 5 mg/mL BSA, 0.05 % sodium azide, 30 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène

    CENPA (Centromere Protein A (CENPA))

    Autre désignation

    CENPA / CENP-A

    Sujet

    Name/Gene ID: CENPA

    Synonyms: CENPA, Centromere autoantigen A, Centromere-specific histone, CenH3, Centromere protein A, Centromere protein A, 17  kDa, CENP-A, Centromere protein A (17kD)

    ID gène

    1058

    UniProt

    P49450

    Pathways

    Chromatin Binding, Maintenance of Protein Location
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