SMAD2 anticorps (Internal Region)
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- Antigène Voir toutes SMAD2 Anticorps
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
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Épitope
- Internal Region
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp SMAD2 est non-conjugé
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Application
- Western Blotting (WB), ELISA
- Specificité
- This affinity purified antibody is directed against human Smad2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. Also, the antibody is Smad2 specific, and reactivity to other Smad proteins (specifically Smad1, Smad3, Smad4, and Smad7) is not detected in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD).
- Réactivité croisée
- Souris, Rat (Rattus)
- Attributs du produit
- This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad2 (also known as Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad2, hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the
- Purification
- affinity purified
- Stérilité
- Sterile filtered
- Immunogène
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This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad23 protein.
Immunogen Type: Peptide - Isotype
- IgG
- Top Product
- Discover our top product SMAD2 Anticorps primaire
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- Indications d'application
- This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract.
- Commentaires
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Gene Name: SMAD2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.55 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
- Date de péremption
- 3 months
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TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration." dans: JCI insight, Vol. 3, Issue 21, (2018) (PubMed).
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TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration." dans: JCI insight, Vol. 3, Issue 21, (2018) (PubMed).
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- Antigène
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- Autre désignation
- SMAD2 (SMAD2 Produits)
- Synonymes
- anticorps JV18, anticorps JV18-1, anticorps MADH2, anticorps MADR2, anticorps hMAD-2, anticorps hSMAD2, anticorps jv18, anticorps Xmad2, anticorps madh2, anticorps madr2, anticorps XSmad2, anticorps hmad-2, anticorps hsmad2, anticorps jv18-1, anticorps smad-2, anticorps fj43c06, anticorps wu:fj43c06, anticorps 7120426M23Rik, anticorps Madh2, anticorps Madr2, anticorps Smad-2, anticorps mMad2, anticorps MAD homolog 2, anticorps Mothers against DPP homolog 2, anticorps SMAD 2, anticorps Smad2, anticorps SMAD family member 2, anticorps SMAD family member 2-Z, anticorps SMAD family member 2 S homeolog, anticorps SMAD2, anticorps SMAD2Z, anticorps smad2, anticorps smad2.S, anticorps Smad2
- Sujet
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This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad2 (also known as Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad2, hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the
Synonyms: MAD antibody, MAD Related Protein 2 antibody, MGC34440 antibody, Mothers Against Decapentaplegic Homolog 2 antibody - ID gène
- 4087, 5174511
- UniProt
- Q15796
- Pathways
- Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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