alpha Tubulin anticorps (Biotin)

N° du produit ABIN2380025

L’anticorps Lapin Polyclonal anti-alpha Tubulin a été validé pour WB, ELISA et IHC. Il convient pour détecter alpha Tubulin dans des échantillons de Humain.

Aperçu rapide pour alpha Tubulin anticorps (Biotin) (ABIN2380025)

Antigène: alpha Tubulin (TUBA1)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha Tubulin est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-alpha Tubulin anticorps (Biotin)

Réactivité croisée: Boeuf (Vache), Poulet, Chévre, Humain, Souris, Rat (Rattus), Mouton

Réactivité croisée (Details): Calculated cross reactivity: Bo Ch Gt Hu Mo Rt Sh

Attributs du produit: Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) (Biotin)

Purification: Purified by immunoaffinity chromatography.

Immunogène: Synthetic peptide corresponding to aa 425-451 at the C-Terminal of human alpha tubulin.

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1:20,000-1:100,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterile dH2O.

Buffer: Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/mL BSA, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: Tubulin, alpha

Sujet: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (A and B-tubulin). A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric a-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated A-tubulin is referred to as G-tubulin. Another post-translational modification of detyrosinated a A tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This makes an excellent loading control.

Pathways: Dynamique des Microtubules