STIM1 anticorps (C-Term, Cytoplasmic Domain)

N° du produit ABIN238397

L’anticorps Souris Monoclonal anti-STIM1 a été validé pour WB, IP, IHC (p) et IF. Il convient pour détecter STIM1 dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour STIM1 anticorps (C-Term, Cytoplasmic Domain) (ABIN238397)

Antigène: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp STIM1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: CDN3H4

Détail du produit anti-STIM1 anticorps

Épitope: C-Term, Cytoplasmic Domain

Specificité: The antibody CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat.

Attributs du produit: Synonyms: Stromal interaction molecule 1

Purification: Protein-A affinity chromatography

Immunogène: Synthetized peptide (C-terminal cytoplasmic part of STIM1)

Isotype: IgG1

Information d'application

Indications d'application: Immunoprecipitation. Western Blotting (reducing and non-reducing conditions): Recommended dilution: 1 μg/mLPositive control: RBL rat basophilic leukemia cell lineSample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing/reducing Laemmli SDS-PAGE sample buffer. Immunocytochemistry: Staining technique: methanol-aceton fixation. Positive control: HeLa human cervix carcinoma cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur STIM1

Antigène: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Autre désignation: STIM1 / GOK

Sujet: STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.Synonyms: Stromal interaction molecule 1

ID gène: 6786

UniProt: Q13586

Pathways: TCR Signaling, BCR Signaling