Clusterin anticorps

N° du produit ABIN238421

L’anticorps anti-Clusterin Monoclonal Souris est utilisé pour la détection de Clusterin dans des échantillons de Humain. Il a été validé pour WB, IHC (p), IF et EIA.

Aperçu rapide pour Clusterin anticorps (ABIN238421)

Antigène: Clusterin (CLU)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Clusterin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: Hs-3

Détail du produit anti-Clusterin anticorps

Specificité: Recognizes Apolipoprotein J (Apo J).

Réactivité croisée (Details): Species reactivity (tested):Human. Negative in Bovine, Canine (Dog) and Feline (Cat).

Attributs du produit: Synonyms: Clusterin, APOJ, CLI, KUB1, AAG4, NA1/NA2, SP-40, 40, Complement-associated proteinSP-40, Complement cytolysis inhibitor, Testosterone-repressed prostate message 2,Ku70-binding protein 1, Aging-associated gene 4 protein

Purification: Affinity Chromatography on Protein A

Immunogène: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% acetic acid and subsequently used forimmunization.

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western blot: 1/ μg/mLPositive Material: seminal plasmaImmunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 μg/mL. Positive Tissue: Sertoli cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4 with 15 mM Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Clusterin

Antigène: Clusterin (CLU)

Autre désignation: Apolipoprotein J (Apo J)

Sujet: Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

ID gène: 1191

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling