S100A9 anticorps

N° du produit ABIN238498

Détail du produit anti-S100A9 anticorps

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A9 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Monoclonal antibody S32.2 identifies the Ca2+ -binding 14 kDa subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. The antigen is MRP14, the epitope is suspected in the carboxyterminal portion of the peptide. The antibody is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14/S100A9 differently depending on the inflammatory status of the disease. Antigen Distribution Isolated Cells: The antigen is found in granulocytes, stimulated monocytes and macrophages. It is absent from all other blood cells. In cultured monocytes, maximum MRP14 expression is found after 3-4 days. Myeloid leukaemia cells have been found to be positive as well. Tissue Sections: MRP14/S100A9 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP14 increasingly during the early stages of inflammation. A high MRP14 (and low MRP8) expression by macrophages was reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases like chronic rheumatoid arthritis, MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/14 determined by antibody 27E10 (Cat.-No. BM4025).Antigen Distribution Does not react with Bovine and Swine spleen.

Purification: Affinity Chromatography.

Immunogène: Cultured human monocytes.

Clone: S32-2

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/400). Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/200), pre-treatment for antigenretrieval not required. Has been described to work in FACS and Dot blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: Stock solution conatins PBS, pH 7.2 with 10 mg/mL BSA, 0.05 % Luviskol as stabilizer and 0.01 % Thimerosal as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Références pour anticorps anti-S100A9 (ABIN238498)

Mortezai, Harder, Schnabel, Moors, Gauly, Schlüter, Wagener, Buck: "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids." dans: Journal of proteome research, Vol. 9, Issue 12, pp. 6126-34, (2010) (PubMed).

Rosenberger, Thorey, Werner, Boukamp: "A novel regulator of telomerase. S100A8 mediates differentiation-dependent and calcium-induced inhibition of telomerase activity in the human epidermal keratinocyte line HaCaT." dans: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6126-35, (2007) (PubMed).

Détails sur S100A9

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Autre désignation: S100A9 / Calgranulin-B / MRP14 (S100A9 Produits)

Synonymes: anticorps 60B8AG, anticorps CAGB, anticorps CFAG, anticorps CGLB, anticorps L1AG, anticorps LIAG, anticorps MAC387, anticorps MIF, anticorps MRP14, anticorps NIF, anticorps P14, anticorps Mrp14, anticorps S100A9, anticorps MRP-14, anticorps p14, anticorps 60B8Ag, anticorps AW546964, anticorps BEE22, anticorps Cagb, anticorps GAGB, anticorps L1Ag, anticorps S100 calcium binding protein A9, anticorps S100 calcium binding protein A9 (calgranulin B), anticorps S100A9, anticorps S100a9

Sujet: S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins. S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.Synonyms: CAGB, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, MRP-14, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, S100-A9

ID gène: 9606

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins