YY1 anticorps

N° du produit ABIN2443872

Cité dans 1 publication. Cet anticorps anti-YY1 Polyclonal Poulet (ABIN2443872) détecte spécifiquement YY1 dans WB et IF. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Mouton et Porc.

Aperçu rapide pour YY1 anticorps (ABIN2443872)

Antigène: YY1 (YY1 Transcription Factor (YY1))

Reactivité: Humain, Souris, Rat, Mouton, Porc

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp YY1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-YY1 anticorps

Fonction: YY1 Chicken Polyclonal Antibody

Specificité: The antibody recognizes the Transcription Factor YY1, in WB, the antibody recognizes the O-glycosylated, the phosphorylated, and the post-translationally unmodified protein in cellular and tissue lysates.

Purification: Purified

Immunogène: Recombinant human YY1

Information d'application

Indications d'application: WB 1:3000,IF 1:500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 30 μL: WB 1:3000 - 1:8000, IF 1:500 - 1:1000 (in tissue paraffin sections). Optimal dilution has to be determined by the user.

Buffer: Lyophilized purified total IgY in PBS pH 7.4

Agent conservateur: Sodium azide, Streptomycin

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Références pour anticorps anti-YY1 (ABIN2443872)

Gelev, Zabolotny, Lange, Hiromura, Yoo, Orlando, Kushnir, Horikoshi, Paquet, Bachvarov, Schaffer, Usheva: "A new paradigm for transcription factor TFIIB functionality." dans: Scientific reports, Vol. 4, pp. 3664, (2015) (PubMed).

Détails sur YY1

Antigène: YY1 (YY1 Transcription Factor (YY1))

Autre désignation: YY1

Sujet: YY1 is a ubiquitous and multifunctional zinc-finger transcription factor involved in the activation or repression of a large number of genes that are critical for basic biological processes of cell growth, development, differentiation, cell cycle and even programmed cell death (apoptosis). YY1 is an essential protein, deletion of YY1 leads to lethality in mouse embryos and disruption of one allele causing developmental defects.

UniProt: P25490