PRMT5 anticorps

N° du produit ABIN2462040

Cet anticorps anti-PRMT5 Polyclonal Lapin (ABIN2462040) détecte spécifiquement PRMT5 dans WB, IHC et ELISA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour PRMT5 anticorps (ABIN2462040)

Antigène: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRMT5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-PRMT5 anticorps

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT5.

Information d'application

Indications d'application: PRMT5 antibody can be used for detection of PRMT5 by ELISA at 1:62500. PRMT5 antibody can be used for detection of PRMT5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PRMT5 antibody at -20 °C.

Détails sur PRMT5

Antigène: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Autre désignation: PRMT5

Sujet: PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription.

Poids moléculaire: 68 kDa, 73 kDa

ID gène: 10419

NCBI Accession: NP_001034708

UniProt: O14744

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development